The Confederation of African Football (Caf) took action after a complaint from Tanzanian side Simba about the eligibility of Janvier Besala Bokungu.
Mazembe beat Simba 6-3 on aggregate in the second round of the tournament.
Simba will play Morocco's Wydad Casablanca, who lost to Mazembe in the third round, for a place in the group stages of the tournament.
The one-off game will be played at a neutral venue next week.
Mazembe have won the Champions League for the last two years and reached the final of the Club World Cup last season, losing 3-0 to Italians Inter Milan in the final
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